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. 2018 Nov 22;9:670. doi: 10.3389/fendo.2018.00670

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Copyright © 2018 Türker, Garreis, Khajavi, Reinach, Joshi, Brockmann, Lucius, Ljubojevic, Turan, Cooper, Schick, Reinholz, Pleyer, Köhrle and Mergler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confirmation of HCK identity. (A) The green anti-LUM antibody immunofluorescent (IF) staining is delimited in the endoplasmic reticulum (arrows). (B) Anti-α-SMA antibody IF staining pattern. Their green staining pattern is less intense. Approximately 20–30% of the HCK cells have no IF signal (arrow heads). (C) IF green staining with anti-KTN antibody detected in all HCKs. (D) A negative control (Ctrl) omitted primary antibody and does not provide a specific IF signal. In all experiments, cell nuclei were counterstained with DAPI (blue) and pictures were merged. Pictures are representative of IF results from five different assays (n = 5).