Figure 12.

3-T₁AM suppresses VEGF-induced increases in whole-cell currents in HCK. (A) Time course recording showing the current increases induced by 10 ng/ml VEGF and current density levels after adding 3-T₁AM (1 μM). There was a delayed increase and decrease of whole-cell currents, respectively. (B) Original traces of VEGF-induced current responses to voltage ramps. Current densities are shown before application (black labeled as A), during application of VEGF (green labeled as B), and after addition of 3T₁AM (red labeled as C). Current densities as function of voltage were derived from the traces shown in (A). Notably, there was a decrease of VEGF-induced in- and outward currents in the presence of 3-T₁AM. (C) Whole-cell currents under control conditions induced by depolarization from −60 to 130 mV in 10 mV steps (400 ms). (D) Increased whole-cell currents (green traces) in the presence of 10 ng/ml VEGF. (E) Decreased whole-cell currents (red traces) in the additional presence of 1 μM 3-T₁AM. (F) Effect of VEGF and 3-T₁AM summarized in a current/voltage plot [I-V plot, data obtained from (C–E)]. The black trace (filled circles) was obtained under control conditions. The green trace was obtained in the presence of 10 ng/ml VEGF and the lower red trace in the additional presence of 3-T₁AM. An inhibitory effect could be observed. (G) Summary of the experiments with VEGF and 3-T₁AM. The asterisks (^*) indicate statistically significant differences of in- and outward currents with and without 3-T₁AM (n = 16; p < 0.05 at the minimum; paired tested).