Figure 7.

Confirmation of functional TRPM8 expression in HCK. The pharmacological changes were carried out at the time points indicated by arrows. Data are mean ± SEM of 5-20 experiments. (A) Menthol (500 μM) induced an irreversible increase in Ca²⁺ influx (n = 20) whereas non-treated control cells showed a constant Ca²⁺ baseline (n = 15). (B,C) Same experiments as shown in (A), but in the presence of BCTC (B), and AMTB (C). Both AMTB and BCTC (both 20 μM) clearly suppressed the menthol-induced Ca²⁺ increase (n = 5–14). (D) Summary of the experiments with menthol, AMTB, and BCTC. The asterisks (^*) designate significance difference between with and without menthol, a TRPM8 agonist (n = 5–20; p < 0.05 at the minimum; paired tested). The hashtags (#) indicate statistically significant differences of fluorescence ratios with and without BCTC or AMTB (p < 0.05 at the minimum; unpaired tested).