Table 3.
Summary of results from next-generation sequencing targeting urinary exosome-derived RNA in cats.
| RNA | NR urine (%) | KD urine (%) | ||||||
|---|---|---|---|---|---|---|---|---|
| miRNA | 0.16 | 0.19 | ||||||
| tRNA | 89.98 | 87.50 | ||||||
| Exonic RNA | 0.65 | 0.45 | ||||||
| Intronic RNA | 0.85 | 0.72 | ||||||
| Others | 8.36 | 11.14 | ||||||
| Accession | Gene ID |
NR urine (read number) |
KD urine (read number) |
Fold change (KD vs. NR) |
Regulation (KD vs. NR) |
Kidney* (miR-X-3p) |
Kidney* (miR-X-5p) |
Description |
| miR-3107 | MI0014103 | 261 | 1818 | 7.0 | up | - | - | same as miR-486b-5p |
| miR-486 | MI0003493 | 333 | 1225 | 3.7 | up | - | + | miR-486a-5p, miR-486b-5p |
| miR-10b | MI0000221 | 7382 | 16319 | 2.2 | up | + | – | |
| miR-143 | MI0000257 | 208 | 88 | −2.4 | down | – | – | |
| miR-27b | MI0000142 | 427 | 171 | −2.5 | down | + | – | |
| miR-30e | MI0000259 | 269 | 96 | −2.8 | down | – | – | |
| miR-6236 | MI0021583 | 256 | 90 | −2.8 | down | NA | NA | |
| let-7f | MI0000563 | 239 | 71 | −3.4 | down | low | + | |
| miR-26a | MI0000706 | 179 | 51 | −3.5 | down | low | + | |
| miR-146a | MI0000170 | 242 | 62 | −3.9 | down | - | - | |
| miR-21a | MI0000569 | 605 | 145 | −4.2 | down | - | - | |
| miR-92a | MI0000719 | 234 | 52 | −4.5 | down | - | - | |
| miR-181a | MI0000697 | 398 | 66 | −6.0 | down | low | + | |
| miR-22 | MI0000570 | 1275 | 160 | −8.0 | down | + | low | |
| miR-378a | MI0000795 | 501 | 49 | −10.2 | down | – | – | |
| miR-142 | MI0000167 | 1758 | 130 | −13.5 | down | low | low |
NR, normal renal function group; KD, kidney disease group.
A total of 241 genes were annotated as miRNAs. NR: normal renal function (n = 43) KD: kidney disease (n = 45). After trimming procedures in NGS, 19,701,266, and 21,499,030 reads were obtained from NR and KD groups, respectively. A total read numbers of 18,704 and 23,874 corresponded to miRNAs from NR and KD groups respectively. One pooled sample was analyzed in each group. Down-regulated genes in KD, genes showing read number > 150 in NR were selected. Up-regulated genes in KD, genes showing read number >150 in KD were selected. For fold change between the 2 groups, genes showing absolute values >2.0 were selected. *Kidney expression information was referred from Ichii et al., Res Vet Sci. 2014, 96(2):299-303. -: not expressed. low: read number <4,000. +: read number >4,000. NA: not available.