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. 2018 Nov 22;8:404. doi: 10.3389/fcimb.2018.00404

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Copyright © 2018 Morgan, Lam, Delgado, Luu, Mohammadi, Isberg, Wang and Auerbuch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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T3SS gene mRNA levels under low and high calcium conditions. Wildtype Y. pseudotuberculosis was grown under high or low calcium conditions in the presence of 50 μM compound or equivalent volume of DMSO and mRNA levels of T3SS (lcrF, yscN, yscD, yscF, lcrV, yopE, yopK, yopH) and non-T3SS genes (L9, erpA) assessed by qPCR. The average of four biological replicates ± standard deviation is shown and statistical significance is represented comparing compounds relative to the DMSO control for each gene. ****P < 0.0001, ***P < 0.0005, **P < 0.003; *P < 0.022 (one way ANOVA with Dunnett's post-hoc test). ns, not significant.