Figure 8.

The size, number and motility of GFP-LAP CHMP2B^Intron5 puncta is not altered in TMEM106B ASO-treated neurons. (A) Representative images of neurons transfected with GFP-LAP CHMP2B^Intron5 and treated with 5 µM of the indicated ASOs. Scale bar = 10 µm. (B) Quantification of the size of GFP puncta, and quantification of the number of GFP puncta per 50 µm section of proximal dendrite in fixed neurons. n = 3 with 3–10 DIV 10 neurons per n. (C) Quantification of the number of motile structures in live-imaged GFP-LAP CHMP2B^Intron5 transfected cells treated with the indicated ASOs. n = 3 with five DIV 10–11 neurons per n.