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. 2018 Nov 28;3(6):e00478-18. doi: 10.1128/mSphere.00478-18

FIG 6.

FIG 6

Promotion of the lytic cycle by EZH2-KO in Akata cells. (A) Increased viral DNA replication in EZH2-KO. WT and EZH2-KO cells latently infected with EBV were inoculated with anti-IgG to induce the lytic cycle. Two days after treatment with anti-IgG, the cells were harvested for DNA extraction, and comparisons of viral and host DNA levels were examined by qPCR. The average and SD from 3 independent replicates are shown. The value of the WT day 0 sample (leftmost bar) was set as 1. (B) Progeny virus production was increased by EZH2-KO. The WT and EZH2-KO cells latently infected with EBV were inoculated with anti-IgG to induce the lytic cycle. Two days after treatment with anti-IgG, viral particles present in the medium were harvested and used to infect to Akata(−) cells. As the EBV genome encodes GFP, infected Akata(−) cells become GFP positive. The average and SD of the GFP-positive ratio (%) from 3 independent replicates are shown. Student’s t test was performed. *, P < 0.001; **, P < 0.0005.