Figure 4.

Aggressive mechanical stimulation reduces calcium ion and P-eNOS levels. (A) Cardiomyocytes cell pellets were collected and lysed in lysis buffer after 24 h stretching, cytosolic calcium ion determined by colorimetric detection kit. (B) Calcium ions released into the medium was measured immediately after stretching at 0 min, 30, 60, and 120 min. The medium which was the supernatant were collected simultaneously, absorbance measured at 575 nm. (C) Calcium ion released into the medium was measured after 24 h stretching with Piezo1 inhibited (Piezo1 inhibitor GsMTx4) at 5%, and 25%. (D) After 21 h of stretching, the cardiac drug simvastatin, or GsMTx4 was added to the cells, and continued cultured for 3 h before collecting cell lysates at 24 h. Data represent at least three independent experiments in SEM ± SD. *, p value < 0.05, **, p value < 0.01, ***, p value < 0.001.