Figure 3.

ABT-263 promotes autophagy. Human PASMCs were treated with DMSO (0.1%) or ABT-263 (1 µM) for 24 h. (A) Cell lysates were subjected to Western blotting to monitor the LC3B-II and p62 levels. The bar graph represents the mean ± standard error of the mean (SEM) of the protein levels determined by densitometry expressed in an arbitrary unit (au). * denotes that the value was significantly different from the DMSO control at p < 0.05 (n = 3). (B) Autophagy was assessed by the detection of autophagic vacuoles with a green fluorescent compound that was incorporated into multilamellar bodies.