Table 1.

Immortalized hypothalamic cell line characteristics.

Cell line	Pnx	Gpr173	Esr1	Esr2	Gpr30	Agrp	Histone 3a
mHypoE-46 (M)	+++	++	++	++	+++	++	++++
mHypoA-2/12 (M)	+++	++	++	+	++	++	++++
mHypoE-41 (F)	+++	++	++	++	++	++	++++
mHypoA-59 (F)	+++	++	++	++	++	++	++++

The cell lines express Pnx and its receptor, Gpr173, as well as other receptors and neuropeptides, for example, the estrogen receptors Esr1, Esr2, and Gpr30, and agouti-related peptide (Agrp). Gene expression was assessed by RT-qPCR and the relative expression is represented by the ranges: ++++CT < 20.0, +++20.0 < CT < 25.0, ++ 25.0 < CT < 30.0, + 30.0 < CT < 35.0, - CT > 35.0. The “E” or “A” within the name denotes they are derived from embryonic or adult mice, respectively, and (M) or (F) denotes male or female.