Table 2.

Effects of the replacement of forage fiber with NFFS in diets on rumen fermentation and biogas production in vitro (n = 9).

Items	Treatment (f NDF/%)1		SEM2	P
	CON(24.0)	TRT(15.8)
pH	6.73	6.72	0.006	0.3782
DMD3	0.8227	0.8450	0.00440	0.0007
NDFD4	0.7717	0.8296	0.00923	0.0007
ADFD5	0.7492	0.8169	0.01074	0.0005
TVFA/(mmol/L)6	77.14	77.56	0.792	0.6896
Acetate/(mmol/L)	47.72	47.91	0.563	0.7966
Propionate/(mmol/L)	17.90	18.30	0.140	0.0146
Isobutyrate/(mmol/L)	0.73	0.74	0.020	0.9023
Butyrate/(mmol/L)	8.57	8.43	0.119	0.5361
Isovalerate/(mmol/L)	1.32	1.28	0.038	0.4917
Valerate/(mmol/L)	0.90	0.90	0.021	0.8978
Acetate/Propinate	2.60	2.62	0.029	0.2347
Total gas production/(mL)	77.56	72.56	4.829	0.6258
H2/(μg)	11.37	12.42	0.481	0.2526
CH4/(mg)	6.26	5.38	0.232	0.0789

¹CON, control diet, a basic total mixed ration; TRT, treatment diet, a modified total mixed ration with replacement of alfalfa by wheat bran and soybean hull.

²SEM, standard error of the mean.

³DMD, dry matter disappearance.

⁴NDFD, neutral detergent fiber disappearance.

⁵ADFD, acid detergent fiber disappearance.

⁶TVFA, total valid fatty acid.