Table 3.

Alpha diversity indices of archaea and bacteria among treatments in vitro (n = 9).

Items	Treatment (f NDF/%)1		SEM2	P
	CON(24.0)	TRT(15.8)
Archaea
OTU3	1127	1093	25.0	0.5057
Coverage	0.99	0.99	0.001	0.3506
Chao1	2042	1888	46.7	0.0946
ACE4	2631	2625	171.6	0.9890
Shannon	2.30	2.25	0.030	0.3591
Simpson	0.25	0.27	0.009	0.0951
Bacteria
OTU	4062	4131	54.3	0.5868
Coverage	0.98	0.98	0.001	0.7395
Chao1	5978	5983	79.1	0.9802
ACE	6926	6787	134.2	0.6396
Shannon	6.46	6.51	0.019	0.0954
Simpson	0.005	0.005	0.0001	0.5094

¹CON, control diet, a basic total mixed ration; TRT, treatment diet, a modified total mixed ration with replacement of forage fiber by wheat bran and soybean hull.

²SEM, standard error of the mean.

³OTU, operational taxonomic units.

⁴ACE, abundance-based coverage estimator.