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. 2018 Nov 20;9:676. doi: 10.3389/fendo.2018.00676

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Copyright © 2018 Liu, Wang, Gao, Jiao and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental design. Timed-pregnant Wistar rats were fed a low-protein diet (undernourished group) or normal diet (control group) from day 0 of pregnancy until full term. A subset of rats was killed at E20 and foetal livers were collected for qPCR array, periodic acid-Schiff (PAS) and immunohistochemical (IHC) staining, and western blotting (WB) studies. Another subset of offspring was delivered spontaneously, male offspring were sacrificed at 3 and 12 W, and the median lobes of their livers were collected for WB and qPCR analysis. LP, low protein; BW, birth weight; IUGR, intrauterine growth restriction; FBG, fast blood glucose.