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. 2018 Nov 19;9:2800. doi: 10.3389/fmicb.2018.02800

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Copyright © 2018 Barbey, Chane, Burini, Maillot, Merieau, Gallique, Beury-Cirou, Konto-Ghiorghi, Feuilloley, Gobert and Latour.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Increased qsdA and qsdC mRNA levels in a ΔqsdR mutant strain. qRT-PCR analysis shows the qsdA and qsdC mRNA levels in the ΔqsdR mutant and in the complementing strain (ΔqsdR-qsdR) grown in 7H9 medium at the mid-exponential (10 h) and stationary (19 h) phases. These mRNA levels are relative to the wild type strain. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist^TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C_T (2^-ΔΔCT) method. ^∗p-value < 0.05; ns means no significant difference.