Figure 6.

A high expression of α_M on resident macrophages reduces their amoeboid migration. (A) The expression of integrin α_D and α_M on resident macrophages was detected with anti-α_D and anti-α_M antibodies, respectively, and tested by flow cytometry analysis. (B) Migration of peritoneal resident macrophages in 3-D fibrin matrix. Migrating resident macrophages from WT and ${\alpha }_{\text{D}}^{-/-}$ mice are shown in the left panel. The middle and right panels represent the migrating resident macrophages from WT and ${\alpha }_{\text{M}}^{-/-}$ mice with or without Rock inhibitor (Y27632). Migrating cells were detected by a Leica Confocal microscope (Leica-TCS SP8). Scale bar = 500 μm. (C) The results were analyzed by IMARIS 8.0 software. Statistical analyses were performed using Student's paired t-tests (n = 4 per group). Data are presented as mean ± SEM. *P < 0.05.