Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 16;9:2725. doi: 10.3389/fmicb.2018.02725

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Kluj, Ebner, Adamek, Ziemert, Mayer and Borisova.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Analysis of cytosolic extracts from wild type, ΔmupG and ΔmupGmurQ cells by LC-MS. S. aureus JE2 wild type (wt) and mutant strains were grown in LB medium for 24 h. Macromolecules were removed from the cytosolic fractions by acetone precipitation and soluble extracts were analyzed by LC-MS in negative ion-mode. Representative mass spectra from at least three independent experiments are presented as base peak chromatograms (BPC) (×10⁵ counts per s [cps]) in black and as differential spectra (DS, BPC_mutant – BPC_wt) (×10⁵ [cps]) in red. DS of both mutants revealed the intracellular accumulation of a compound (absent in the wt cells) with an observed mass of (M-H)^- = 575.151 m/z in ΔmupG cells and an observed mass of (M-H)^- = 575.148 m/z) in ΔmupGmurQ cells.