Figure 2.
Identification of extracts from fruit and stem of CJ enhance human somatic cell reprogramming. (a) Schematic experimental procedure of human pluripotent stem cell (hiPSC) generation. The human foreskin fibroblasts (hFFs) were seeded at 1 × 105 cells per well in six-well plates, and cells were transduced with OSKM-Sendai virus (SeV) on the following day. After five days, OSKM-transduced hFFs were reseeded on Matrigel-coated 12-well plates and treated with or without different CJ extracts. (b) The groups of crude extracts from fruits or stems of CJ promote hiPSC generation. The number of AP+ colonies on day 15 post-treatment was used as a measure of the reprogramming efficiency. The relative fold changes in AP+ colonies compared with untreated controls (designated as 1) are presented. (c) The mRNA expression of the pluripotency markers Rex1 (left) and Nanog (right) at reprogramming days 0, 7, and 15 by qPCR. The results are presented as the mean + SEM (n = 3). The statistical significance was determined using Student’s t-test (* p < 0.05, ** p < 0.01, *** p < 0.001).