Figure 2.

Construction of WT, WT_OE, STXC and STXCOE strains. (A) Schematic representation of the trxC and glnN loci in the WT and mutant strains. (B) PCR analysis of the mutant strains using the oligonucleotides indicated in (A). (C) Western-blot analysis of TrxC protein levels in the WT, WTOE, STXC and STXCOE strains. Cells were grown in BG11 supplemented with 1% CO₂ to mid-log growth phase and 1 OD_750nm was collected before and after 2 h of 0.3 µM Cu addition. The pellet was resuspended in 1× Laemmli buffer and boiled for 10 min, then 20 µL of the boiled cell suspension were loaded on and gel and analyzed by western blot.