Table 1.
Antibodies Used for Tissue Stainings and Flow Cytometry.
| Antibody | Clone | Isotype | Company (Cat. No.) | Dilution, Peroxidase | Dilution, Fluorescence | Dilution, Flow Cytometry |
|---|---|---|---|---|---|---|
| CAM5.2 | CAM5.2 | IgG2a | BD Biosciences (345779) | 1:25 | 1:10 | — |
| CD117 | K45 | IgG2a | Thermo Scientific (MS-289-P) | 1:100 | 1:50 | — |
| CD117 | 104D2 | IgG1 | Aviva Systems (OAEE00056) | 1:100 | 1:50 | — |
| CD326 | 9C4 | IgG2b | BioLegend (324202) | — | 1:25 | — |
| ERα | 1D5 | IgG1 | Dako (M7047) | 1:50 | — | — |
| ERα | Sp1 | Rabbit | Thermo Scientific (RM-9101-S) | 1:50 | — | |
| GATA3 | HG3-31 | IgG1 | Santa Cruz (sc-268) | 1:200 | 1:50 | — |
| Ki-67 | MIB-1 | IgG1 | Dako (M7240) | 1:100 | 1:50 | — |
| Ki-67 | Sp6 | Rabbit | Thermo Scientific (RM-9106-S) | 1:100 | 1:25 | — |
| CD117-PE | 104D2 | IgG1 | BD Biosciences (332785) | — | — | 1:20 |
| CD166-Alexa Fluor 488 | 3A6 | IgG1 | AbD Serotec (MCA1926A488) | — | — | 1:20 |
| CD271-APC | ME20.4 | IgG1 | Cedarlane (CL10013APC) | — | — | 1:50 |
| CD326-PerCP/Cy5.5 | 9C4 | IgG2b | BioLegend (324214) | — | — | 1:20 |