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. 2018 Nov 15;8(11):209. doi: 10.3390/ani8110209

Table 3.

Terms used by observers to describe sheep behavioural expression during road transport. Terms showing the strongest correlations (r > 0.5) with low and high values on each Generalised Procrustes Analysis (GPA) dimension of the consensus profile (maximum 10 terms presented). Order of terms is determined firstly by number of observers to use that term (in parentheses where >1) and secondly by weighting of each term.

Treatment, Procrustes Statistic GPA Dimension Low Values High Values Treatment Effect
Study A
A1: S-C
PS: 56.64% (t99 = 43.8, p < 0.001)
1
(53.2%)
Agitated (7), anxious (5), nervous (5), worried (5), concerned (3), scared (3), jumpy (2), fidgety (2), alert (2), distressed (2) Calm (11), relaxed (7), settled (3), restful (2), comfortable (2), subdued, happy, tolerant, accepting, bored Crate: F1, 6 = 48.85, p < 0.001
Sheep in a standard crate scored higher than sheep in a convertible crate
Driver ID (random factor): F6, 487 = 5.90, p < 0.001
2
(8.5%)
Curious (7), alert (3), inquisitive (2), nervous (2), interested, confident, comfortable, puzzled, at ease, watchful Frightened, frustrated, agitated, bored, annoyed, distressed, anxious, bossy Crate: F1, 6 = 0.29, p = 0.609
Driver ID (random factor): F6, 487 = 26.84, p < 0.001
3
(4.8%)
Tired (2), apprehensive, struggling, pissed off, sad, fidgety, certain, alert, sleepy, bored Confident, relaxed, sleepy, depressed, settled, satisfied Crate: F1, 6 = 0.96, p = 0.367
Driver ID (random factor): F6, 487 = 6.67, p < 0.001
A2: M-FT
PS: 56.11% (t99 = 12.01, p < 0.001)
1
(42.5%)
Agitated (6), distressed (6), nervous (5), anxious (4), jumpy (3), pushy (2), alert (2), wary (2), frustrated (2), fidgety (2) Calm (6), relaxed (4), comfortable (4), happy (3), settled (2), patient (2), composed (2), restful, mellow, unphased Breed: F1, 4 = 27.98, p = 0.006
Merino sheep scored higher than fat-tail sheep
Driver ID (random factor): F2, 223 = 30.87, p < 0.001
2
(12.6%)
Curious (5), alert (4), inquisitive (4), worried (3), interested (2), comfortable (2), nervous (2), attentive, aware, confident Tired (2), content (2), happy, calm, scared, settled, annoyed, anxious, frustrated, nervous Breed: F1, 14 = 8.44, p = 0.011
Merino sheep scored lower than fat-tail sheep
Driver ID (random factor): F2, 223 = 7.30, p = 0.001
3
(9.9%)
Nervous (3), alert (3), interested (2), curious (2), annoyed (2), calm (2), jumpy, nonchalant, anticipating, restless Relaxed (3), tired (2), alert (2), anxious (2), scared (2), comfortable (2), sleepy (2), unsure, insecure, resigned Breed: F1, 3 = 0.05, p = 0.837
Driver ID (random factor): F2, 223 = 75.96, p < 0.001
A3: FL-SY
PS: 49.2% (t99 = 29.051, p < 0.001)
1
(46.6%)
Agitated (4), anxious (4), frightened (3), worried (3), nervous (3), panicked (3), scared (2), restless (2), jumpy, fretful Calm (6), bored (3), relaxed, casual, accepting, placid, comfortable, settled, patient, composed Origin: F1, 6 = 0.12, p = 0.745
Driver ID (random factor): F5, 469=27.51, p < 0.001
2
(10.7%)
Curious (3), happy (2), alert (2), bored (2), interested (2), comfortable, calm, settled, resigned, content Curious (2), agitated (2), resigned, interested, tired, lethargic, depressed, struggling, distressed, bored Origin: F1, 18 = 1.12, p = 0.304
Driver ID (random factor): F5, 469=4.21, p < 0.001
3
(5.8%)
Nervous (2), curious (2), distressed, excited, interested, agitated, annoyed, alert Comfortable (2), relaxed (2), aware, stressed, mellow, calm, settled, confident, happy, agitated Origin: F1, 24 = 7.04, p = 0.014
Sheep from feedlot scored higher than sheep from saleyard
Driver ID (random factor): F5, 469 = 3.11, p = 0.009
Study B
B1: SU-SL
PS: 42.75% (t99 = 16.45, p < 0.001)
1
(35.9%)
Agitated (7), stressed (4), pushy (3), distressed (3), worried (3), uneasy (2), restless (2), nervous (2), scared(2), distressed (2) Calm (7), comfortable (4), relaxed (2), happy (2), placid, quiet, accepting Deck (S): F1, 377 = 0.41, p = 0.521
Driver ID (random factor): F2, 377 = 15.03, p < 0.001
2
(20.9%)
Miserable, unsure, tired, restful Alert (9), curious (6), interested (5), happy (3), inquisitive (2), watchful (2), comfortable (2), observant, confused, confident Deck (S): F1, 377 = 136.22, p < 0.001
Sheep transported in the upper deck scored higher than sheep transported in the lower deck
Driver ID (random factor): F2, 377 = 6.04, p = 0.003
3
(10.8%)
Nervous (2), agitated (2), stressed, distressed, settled, weary, wary, dejected, collected, confused Stressed (2), alert (2), restless (2), distressed, adjusting, anxious, quiet, fidgety, unsupported, relaxed Deck (S): F1, 377 = 25.40, p < 0.001
Sheep transported in the upper deck scored higher than sheep transported in the lower deck
Driver ID (random factor): F2, 377 = 4.21, p = 0.015
B2: CU-CL
PS: 45.34% (t99 = 23.67, p < 0.001)
1
(56.1%)
Agitated (6), stressed (5), nervous (4), frightened (4), restless (4), distressed (3), uneasy (3), anxious (2), unsettled (2), worried (2) Calm (9), relaxed (7), comfortable (6), happy (3), quiet (2), resolved, settled, at ease, resigned, restful Deck (C): F1, 376 = 22.54, p < 0.001
Sheep transported in the upper deck scored higher than sheep transported in the lower deck
Driver ID (random factor): F3, 376 = 32.58, p < 0.001
2
(8.5%)
Dejected, weary, agitated, tired, scared Alert (6), curious (4), interested (2), confident (2), relaxed (2), happy, wary, observant, aware Deck (C): F1, 376 = 22.54, p < 0.001
Sheep transported in the upper deck scored higher than sheep transported in the lower deck
Driver ID (random factor): F3, 376 = 32.58, p < 0.001
3
(7.8%)
Nervous, worried, unsure Agitated (2), cramped (2), curious (2), alert, aware, interested, relaxed, squashed Deck (C): F1, 376 = 49.17, p < 0.001
Sheep transported in the upper deck scored higher than sheep transported in the lower deck
Driver ID (random factor): F3, 376 = 24.54, p < 0.001
B3: SU-SL-CU-CL
PS: 36.86% (t99 = 32.699, p < 0.001)
1
(43.2%)
Agitated (5), nervous (3), restless (3), uneasy (3), frightened (3), anxious (2), stressed (2), worried (2), distressed (2), confused (2) Calm (7), relaxed (5), comfortable (4), happy, at ease, settled Crate: F1, 5 =0.35, p = 0.581
Deck: F1, 6 = 0.01, p = 0.968
Crate x deck: F1, 5 = 0.63, p = 0.466
Driver ID (random factor): F5, 5 = 4.30, p = 0.065
2
(19.1%)
Distressed (2), stressed (2), bored, scared, freaked, miserable, quiet, unsure, worried, sad Curious (8), alert (7), aware (3), interested (2), observant (2), confident, happy, relaxed Crate: F1, 6 = 0.47, p = 0.525
Deck: F1, 8 = 12.35, p = 0.008
Sheep transported in the upper deck scored higher than sheep transported in the lower deck
Crate x deck: F1, 5 = 0.51, p = 0.509
Driver ID (random factor): F5, 5 = 1.42, p = 0.355
3
(8.8%)
Nervous (2), curious (2), distressed, excited, interested, agitated, annoyed, alert Quiet (2), collected, calm, confused, accepting, aloof, peaceful Crate: F1, 5 < 0.01, p = 0.994
Deck: F1, 4 = 4.50, p = 0.114
Crate x deck: F1, 4 = 0.82, p = 0.412
Driver ID (random factor): F5, 5 = 2.02, p = 0.222

PS: Procrustes Statistic showing the percentage of variance in the dataset that could be attributed to the consensus in scoring between individual observers and the results for the one-way t-test comparing this result with a mean randomised profile of the same dataset, indicating that the consensus between observers in their use of descriptive terms to quantify the behavioural expression of these sheep was statistically significant.