CircFGFR2 promotes myoblast proliferation. (A,B) The relative expression of circFGFR2 after transfected chicken primary myoblasts with 1 µg pCD2.1-circFGFR2 or 50 nM si-circFGFR2 for 48 h. (C,D) The relative expression of circFGFR2 after transfected QM-7 cells with 1 µg pCD2.1-circFGFR2 or 50nM si-circFGFR2 for 48 h. (E,F) Cell cycle analysis of chicken primary myoblasts transfected with 1 µg circFGFR2 pCD2.1-circFGFR2 or 50 nM si-circFGFR2 for 36 h. (G) 5-ethynyl-2′-deoxyuridine (EdU) assays for chicken primary myoblasts transfected with 1 µg circFGFR2 pCD2.1-circFGFR2 or 50 nM si-circFGFR2 for 36 h. (H,I) The percentage of EdU-stained chicken primary myoblasts after overexpression or knockdown of circFGFR2 for 36 h. (J,K) Cell cycle analysis of QM-7 cells transfected with 1 µg circFGFR2 pCD2.1-circFGFR2 or 50 nM si-circFGFR2 for 48 h. (L) EdU assays for QM-7 cells transfected with 1 µg circFGFR2 pCD2.1-circFGFR2 or 50 nM si-circFGFR2 for 48 h. (M,N) The percentage of EdU-stained chicken primary myoblasts after overexpression or knockdown of circFGFR2 for 48 h. In all panels, the results are shown as mean ± S.E.M., and the data are represented by three independent assays. Statistical significance of differences between means was assessed using an unpaired Student’s t-test (* p < 0.05; ** p < 0.01).