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. 2018 Nov 6;7(11):199. doi: 10.3390/cells7110199

Figure 4.

Figure 4

miR-133a-5p and miR-29b-1-5p inhibit myoblast proliferation. (A,B) The relative expression of miR-133a-5p and miR-29b-1-5p after transfected chicken primary myoblast with 50 nM miR-133a-5p and miR-29b-1-5p mimic for 48 h. (C,D) Cell cycle analysis of chicken primary myoblasts transfected with 50 nM miR-133a-5p and miR-29b-1-5p mimic for 36 h. (E,F) Cell cycle analysis of QM-7 cell transfected with 50 nM miR-133a-5p and miR-29b-1-5p mimic for 48 h. (G,H) EdU assay of chicken primary myoblasts transfected with 50 nM miR-133a-5p or miR-29b-1-5p mimic for 36 h. (I,J) EdU assay of QM-7 cell transfected with 50 nM miR-133a-5p or miR-29b-1-5p mimic for 48 h. In all panels, results are expressed as the mean ± S.E.M. of three independent experiments, and statistical significance of differences between means was assessed using an unpaired Student’s t-test (* p < 0.05; ** p < 0.01). NC, negative control.