Figure 2. SiHa and HeLa cells were cultured in normal medium (Ctrl), then transfected with glycoprotein nonmetastatic melanoma protein B (GPNMB) siRNA or negative control siRNA (s-Ctrl). A, RT-PCR assay was performed to evaluate the transfection efficacy of specific GPNMB siRNA in cervical cancer cells. B, CCK-8 assay was conducted to clarify the proliferation of SiHa and HeLa cells after treatment with siRNA or not. C, Migration capacity of cells evaluated by Transwell Boyden chambers. D, Apoptosis of cells was measured by flow cytometry. Data are reported as means±SE. *P<0.05 vs Ctrl (ANOVA).