Figure 4. Association between Wnt/β-catenin pathway and glycoprotein nonmetastatic melanoma protein B (GPNMB)-mediated cervical cancer tumorigenesis. SiHa and HeLa cells were cultured in normal medium (Ctrl), then transfected with GPNMB siRNA or negative control siRNA (s-Ctrl). A, Expression of nuclear β-catenin measured by western blotting in SiHa and HeLa cells as well as in normal cervical epithelial cells. B, Cells were treated by 100 ng/ml Dkk-1 or 1 nM BIO for 48 h or not, and matrix metalloproteinases (MMP)-2 and MMP-9 expressions were then measured by western blotting. C, CCK-8 assay was conducted to clarify the proliferation of SiHa and HeLa cells after treatment with Dkk-1 or BIO. D, Migration capacity of cells tested by Transwell Boyden chambers. E, Apoptosis of cells measured by flow cytometric analysis. Data are reported as means±SE. ^#P<0.05 vs Normal; *P<0.05 vs Ctrl (ANOVA).