Figure 2. Role of up-regulated high temperature requirement factor A1 (HtrA1) on pancreatic cancer cell growth, apoptosis, and migration. After transfection with pcDNA3.1-HtrA1 plasmids, the expression of HtrA1 was measured by real-time PCR (A) and western blot (B). CCK-8 assay (C) and colony formation assay (D) were performed to detect the cell viability and colony formation of PANC-1 cells. Flow cytometry and Transwell assay were used to evaluate the apoptosis (E) and migration (F) of PANC-1 cells. Data are reported as means±SD. *P<0.05; **P<0.01 (t-test).
