Figure 3. After transfection with high temperature requirement factor A1 (HtrA1)-specific siRNA, real-time PCR (A) and western blot (B) were performed to detect the expression of HtrA1 in PANC-1 cells. Cell viability and colony number were measured by CCK-8 assay (C) and colony formation assay (D), respectively. Data are reported as means±SD. *P<0.05; **P<0.01 (t-test).
