Figure 4. High temperature requirement factor A1 (HtrA1) regulated the expression of Notch-1 in pancreatic cancer cells. After transfection with pcDNA3.1-HtrA1 plasmids, the expression of Notch-1 was determined by real-time PCR (A) and western blot (B). Moreover, PANC-1 cells were transfected with HtrA1-siRNA, and then the Notch-1 transcripts (C) and protein levels (D) were measured by real-time PCR and western blot, respectively. Data are reported as means±SD. **P<0.01 (t-test).
