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. 2018 Nov 16;131(22):jcs220517. doi: 10.1242/jcs.220517

Fig. 2.

Fig. 2.

EGF-induced CDR formation, mTORC1 activity and Akt phosphorylation are inhibited by nocodazole. (A) CDR (arrow, 3 min) and macropinosome formation (arrowheads, 15 min) in response to EGF (16 nM) in MEFs. Scale bar: 10 µm. (B) The frequency of cells inducing CDRs at the indicated times after EGF stimulation. (C) Nocodazole (Noco) treatment blocked EGF-induced CDR formation. (D) Effects of leucine concentration on EGF-induced S6K phosphorylation (mTORC1). Cells were incubated in DPBS containing 0.4 mM or 4.0 mM leucine for 30 min and then stimulated by EGF for 15 min. Phosphorylation of S6K but not Akt or ERK required leucine. (E) Effects of nocodazole treatment on EGF-induced signal pathways. Cells were incubated in DPBS containing 0.4 mM leucine for 30 min with or without nocodazole and then stimulated by EGF for 15 min. Nocodazole treatment inhibited S6K phosphorylation and attenuated Akt phosphorylation without inhibiting EGF-stimulated ERK phosphorylation.