Fig. 8.

GCN5L1-dependent α-tubulin acetylation contributes to lysosomal centrifugal distribution. (A) Lysosomes show abnormal perinuclear clusters in LKO hepatocytes compared to WT hepatocytes. Lysosome distribution was visualized by immunostaining for Lamp1 (green), a lysosome membrane protein, in WT and LKO hepatocytes. The marked regions are magnified in the adjacent panel. (B) The HDAC6 inhibitor (tubacin) increased the acetylation of tubulin in WT and GCN5L1 LKO hepatocytes. This intervention partially restored the peripheral distribution of lysosomes in LKO hepatocytes. Lamp1 (green) and acetylated α-tubulin (Ac-Tub; red) were immunostained in WT and LKO hepatocytes treated with or without tubacin. Magnified views of the marked regions reveal that some lysosomes are delivered to the cell surface region in LKO hepatocytes (arrowheads). DNA was visualized by DAPI staining (blue). Scale bars: 10 µm.