Positive effect of bezafibrate (BZF) on osteogenesis of LS cells. (A) OBs were stained with Alizarin Red-S, which was extracted and measured by absorbance at 570 nm. Data represent the mean ± standard deviation from three experiments. *P < 0.05. (B) The expression of ALP mRNA in OBs was measured by RT-qPCR. The relative difference was calculated, and the level of Ctrl-OB was set as 1. Data represent the mean ± standard deviation from three experiments. *P < 0.05, **P < 0.01. (C) The protein expression of active β-catenin was measured by western blotting. The relative difference was calculated, and the level of Ctrl-OB was set as 1. Data represent the mean ± standard deviation from three experiments. *P < 0.05, ***P < 0.001.