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. 2018 Nov 28;17:32–37. doi: 10.1016/j.bbrep.2018.11.003

Fig. 2.

Fig. 2

Effect of bezafibrate (BZF) on mitochondrial function of LS cells. (A) The intracellular ATP levels in OBs were measured using an ATP luminescence assay. The relative difference was calculated, and the level of Ctrl-OB was set as 1. Data represent the mean ± standard deviation from three experiments. *P < 0.05. (B) Mitochondrial calcium levels were measured by Rhod-2 AM staining. The fluorescence intensity of Rhod-2 AM is shown in the graph. Data represent the mean ± standard deviation from three experiments. *P < 0.05, **P < 0.01. a.u., arbitrary unit. (C) MMP was measured by JC-1 staining. JC-1 red and green fluorescence signals were analyzed by flow cytometry. The relative differences in the ratio of red/green fluorescence were calculated, and the ratio of red/green fluorescence in Ctrl-OB was set as 1. Data represent the mean ± standard deviation from three experiments. *P < 0.05; n.s., not significant.