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. 2018 Nov 28;17:32–37. doi: 10.1016/j.bbrep.2018.11.003

Fig. 3.

Fig. 3

Effect of bezafibrate (BZF) in mitochondrial biogenesis and morphology of LS cells. (A) The expression of PGC-1α in OBs was measured by RT-qPCR. The relative difference was calculated, and the level of Ctrl-OB was set as 1. Data represent the mean ± standard deviation from three experiments. *P < 0.05. (B) The cells were immunostained with anti-Tom20 antibody (a mitochondrial marker), and counterstained with DAPI. Details of the boxed region in the upper panels are shown in the lower panels. Yellow arrows indicate fragmented mitochondria. (C) The number of cells with fragmented mitochondria was counted. The percentage to total cell number was calculated. Data represent the mean ± standard deviation from the analysis of thirty cells from five immunostained images. ***P < 0.001. (D) The fluorescence intensity of Tom20 in the stained area was measured and divided by the number of nuclei for quantitative analysis. Data represent the mean ± standard deviation from the analysis of four immunostained images. ***P < 0.001. (E) DRP1 expression in OBs was analyzed by immunoblotting. DRP1 expression was normalized to α-Tubulin expression. DRP1 expression in Ctrl-OB was set as 1. The mean ± SEM from three experiments is shown. *P < 0.05.