Fig. 1.

Timeline of experimental procedures. Mice were inoculated with PBS (control) or 10⁸ CFU of enterohemorrhagic E. coli (EHEC) 278F2 or non-pathogenic E. coli MG1655. Weekly fecal samples were collected as indicted. The diet was changed to Teklad 2920X for fluorescent imaging and mice harboring the altered Schaedler flora (ASF; n=3 per group) were injected on day 27 post-inoculation with ProSense 680. At necropsy, the gastrointestinal (GI) tract was excised and imaged for mucosal inflammation (ASF mice only), intestinal contents were collected for enumeration of E. coli [ASF and conventionally reared (CONV) mice], and intestinal tissues were harvested (ASF mice only) for histopathology and host transcriptome profiling.