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. 2018 Nov 20;11(11):dmm036699. doi: 10.1242/dmm.036699

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Localization of NDUFA4 within the mitochondrial electron chain complexes. (A,B) Localization of NDUFA4 by denaturing 1D western blot within the mitochondrial electron chain complexes I and IV (A; consisting of a composite of a ladder and two separate blots on the same membrane, one with the anti-NDUFA4 antibody and the other with OXPHOS) and by 2D blue native gels, with a molecular mass of ∼10 kDa for NDUFA4 in complex I and a molecular mass of 75-100 kDa in complex IV (B; also consisting of a composite of two separate blots on the same membrane, one with the anti-NDUFA4 antibody and the other with OXPHOS).