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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Anal Biochem. 2018 Oct 5;564-565:21–31. doi: 10.1016/j.ab.2018.10.001

Figure 4. Kinetic analysis of VEGF165 binding to VEGFR-1 and VEGFR-2 Fc chimeras on an SPR chip.

Figure 4.

VEGFR-1 and VEGFR-2 Fc chimeras were immobilized onto an activated chip surface until reaching 728 and 407 RU of immobilization respectively. Increasing concentrations of recombinant human VEGF165 in running buffer (6.25, 12.5, 25, 50 and 100 nM) were flowed over the surface and the response was measured for 60s at 50 μL/min. Dissociation was measured after switching the flow to running buffer. All sensograms were referenced to a surface containing immobilized FGFR-1 Fc and a blank injection to correct for bulk shifts. Association and dissociation phases were analyzed by non-linear curve fitting (red lines). Parameters obtained by fitting are shown in Tables 2 and 3. Req values were plotted against VEGF concentration to implement equilibrium analysis and results are shown in Table 5.