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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Anal Biochem. 2018 Oct 5;564-565:21–31. doi: 10.1016/j.ab.2018.10.001

Figure 5. Kinetic analysis of VEGF165 binding to NRP-1.

Figure 5.

NRP-1 Fc chimera (A and B) or NRP-1 monomer (C and D) were immobilized onto an SPR chip reaching 295 and 166 RU of immobilization, respectively. VEGF165 (6.25, 12.5, 25, 50 and 100 nM) binding responses were measured for 60 s at 50 μL/min. Association and dissociation phases were analyzed by non-linear curve fitting after correction (red lines). Parameters obtained by fitting are shown on Tables 4 and 5.