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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Anal Biochem. 2018 Oct 5;564-565:21–31. doi: 10.1016/j.ab.2018.10.001

Figure 6. Kinetic analysis of VEGF121 binding to VEGFR-1 and VEGFR-2 Fc chimera on a SPR chip.

Figure 6.

Increasing concentrations (6.25, 12.5, 25, 50 and 100 nM) of recombinant human VEGF121 in running buffer were flowed over the surfaces containing VEGFR-1 and VEGFR-2 Fc chimeras (1610 and 1450 RU immobilized respectively) and response was measured for 180 s at 30 μL/min. Dissociation was measured after switching the flow to running buffer. Association and dissociation phases were analyzed by non-linear curve fitting (red lines). Results of the curve fit are shown in Tables 7–9.