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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Anal Biochem. 2018 Oct 5;564-565:21–31. doi: 10.1016/j.ab.2018.10.001

Table 3.

Analysis of kinetics of VEGF165 binding to VEGFR-2.

A.
[VEGF165]
(nM)		 to (s)
Fixed		 Req ± SE
(RU)		 Rmin ± SE
(RU)		 kd ± SE
(s−1)		 Red.
X2 		Adj.
R2
100 55.8 10.2 ± 0.3 24.6 ± 0.2 1.51 ± 0.07 × 10 −2 3.29 0.97
50 8.8 ± 0.3 21.9 ± 0.1
25 6.4 ± 0.3 18.1 ± 0.1
12.5 3.3 ± 0.3 12.7 ± 0.1
6.25 1.5 ± 0.3 10.2 ± 0.1
B.
[VEGF165] (M)
Fixed		 ka ± SE
(M−1s−1)		 Rmax ± SE
(RU)		 kd – Fixed
(s−1)		 Red.
X2 		Adj.
R2 		KD ± SE
(nM)
1.00 × 10−7 1.76 ± 0.04 × 106 34.9 ± 0.3 1.51 × 10 −2 1.76 0.96 8.6 ± 0.5
5.00 × 10−8
2.50 × 10−8
1.25 × 10−8
6.25 × 10−9

A. Dissociation phase analysis of VEGF165 from VEGFR-2. Parameters obtained from global curve fitting (red lines Figure 4C) of dissociation data from VEGFR-2 Fc chimera (Equation 3). B. Association phase analysis of VEGF165 binding to VEGFR-2. Parameters from fitting association phase data (red lines Figure 4C) and calculated. affinity (KD = kd / ka) from - VEGFR-1 interaction.