Table 4.

Analysis of kinetics of VEGF₁₆₅ binding to NRP-1.

A.
[VEGF165] (nM)	to (s) Fixed	Req ± SE (RU)	Rmin ± SE (RU)	kd ± SE (s−1)	Red. X2	Adj. R2
100	57.6	13.9 ± 0.7	2.3 ± 0.1	9.0 ± 0.4 × 10 −2	1.75	0.66
50		14.2 ± 0.7	2.2 ± 0.1
25		8.8 ± 0.6	1.7 ± 0.1
12.5		6.1 ± 0.6	1.8 ± 0.1
6.25		4.3 ± 0.6	1.7 ± 0.1

B.
[VEGF165] (M) Fixed	ka ± SE (M−1s−1)	Rmax ± SE (RU)	kd – Fixed (s−1)	Red. X2	Adj. R2	KD ± SE (nM)
1.00 × 10−7	3.6 ± 0.2 × 106	21.8 ± 0.4	9.0 × 10 −2	2.27	0.92	25 ± 2
5.00 × 10−8
2.50 × 10−8
1.25 × 10−8
6.25 × 10−9

C.
[VEGF165] (nM)	to (s) Fixed	Req ± SE (RU)	Rmin ± SE (RU)	kd ± SE (s−1)	Red. X2	Adj. R2
100	54.9	22.3 ± 0.6	4.1 ± 0.1	6.5 ± 0.2 × 10−2	1.89	0.80
50		18.8 ± 0.5	2.0 ± 0.1
25		13.1 ± 0.5	2.9 ± 0.1
12.5		8.4 ± 0.5	2.6 ± 0.1
6.25		6.2 ± 0.5	2.5 ± 0.1

D.
[VEGF165] (M) Fixed	ka ± SE (M−1s−1)	Rmax ± SE (RU)	kd – Fixed (s−1)	Red. X2	KD ± SE (nM)
1.00 × 10−7	2.64 ± 0.08 × 106	31.4 ± 0.4	6.5 × 10−2	2.38	25 ± 1
5.00 × 10−8
2.50 × 10−8
1.25 × 10−8
6.25 × 10−9

A. Dissociation phase analysis of VEGF₁₆₅ from NRP-1 Fc chimera. Parameters obtained from global curve fitting (red lines Figure 5A) of dissociation data from NRP-1 Fc chimera (Equation 3). B. Association phase analysis of VEGF₁₆₅ binding to NRP-1 Fc chimera. Parameters from global fitting of association phase data to equation 2 (red lines Figure 3.4A) and calculated affinity (K_D = k_d / k_a). C. Dissociation phase analysis of VEGF₁₆₅ from NRP-1 monomer. Parameters obtained from global curve fitting (red lines Figure 5C) of dissociation data from NRP-1 monomer (Equation 3). D. Association phase analysis of VEGF₁₆₅ binding to NRP-1 monomer. Parameters from global fitting of association phase data to equation 2 (red lines Figure 3.4C) and calculated affinity (K_D = k_d / k_a).