Table 7.

Analysis of kinetics of VEGF₁₂₁ binding to VEGFR-1.

A.
[VEGF121] (nM)	to (s) Fixed	Req ± SE (RU)	kd ± SE (s−1)	Red. X2	Adj. R2
100	174.6	30.1 ± 0.4	8.8 ± 0.2 × 10 −3	6.12	0.86
50		26.2 ± 0.4
25		22.8 ± 0.4
12.5		19.0 ± 0.4
6.25		15.6 ± 0.4

B.
[VEGF121] (M) Fixed	ka ± SE (M−1s−1)	Rmax ± SE (RU)	kd – Fixed (s−1)	Red. X2	Adj. R2	KD ± SE (nM)
1.00 × 10−7	1.43 ± 0.03 × 106	42.6 ± 0.2	8.8 × 10 −3	11.1	0.90	6.2 ± 0.2
5.00 × 10−8
2.50 × 10−8
1.25 × 10−8
6.25 × 10−9

A. Dissociation phase analysis of VEGF₁₂₁ from VEGFR-1. Parameters obtained from global curve fitting (red lines Figure 6A) of dissociation data from VEGFR-1 (Equation 3). B. Association phase analysis of VEGF₁₂₁ binding to VEGFR-1. Parameters from global fitting of association phase data to equation 2 (red lines Figure 6A) and calculated affinity (K_D = k_d / k_a).