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Sample preparation workflows used in the study. Elephant seal blubber samples were divided in half and each was lysed by bead beating with either Qiazol® (QIA method) or buffer containing sodium deoxycholate detergent (SDC method). After protein precipitation from tissue lysates, samples were treated identically (grey boxes). MS/MS data was analyzed by SEQUEST database search in Proteome Discoverer or by de novo sequencing and PEAKS DB database search in PEAKS Studio.
