Fig. 7.

Plagl2 is sufficient to alter the proliferation of neocortical progenitors within 24 h post-electroporation. (A) Schematic representation of gain-of-function experiment using in utero electroporation. (B–Q) E12.5 to E13.5 electroporations of pCIG2 (B,F,J,N), Plag1 (C,G,K,O) and Plagl2 (D,H,L,P) analyzed for the expression of BrdU (B–D), pHH3 (F–H), Pax6 (J–L), Tbr2 (N–P). Quantitation of the ratios of GFP⁺ cells that are BrdU⁺ (E), pHH3⁺ (I), Pax6⁺ (M) and Tbr2⁺ (Q). Error bars are s.e.m. ns, not significant; *P<0.05, **P<0.01, and ***P<0.005. pp, preplate; vz, ventricular zone. Scale bars: 125 μm.