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. 2018 Nov 15;7(11):bio034355. doi: 10.1242/bio.034355

Fig. 1.

Fig. 1.

The availability of integrin β1 on the plasma membrane of hES cells after detachment with different methods. (A) The diagram shows the detachment methods for hES cells. The hES cells were dissociated and detached manually (hESC) or with EDTA with or without following the treatment with Y-27632 or with trypsin. (B) Comparison of integrin β1 levels on the plasma membrane of differently detached hES cells analysed by flow cytometry. The data were collected from seven independent experiments and are presented as mean±s.d. (C) Detection of E-cadherin on the plasma membrane of hES cells analysed by flow cytometry (n=4). (D) Comparison of integrin α6 levels on the plasma membrane of differently detached hES cells analysed by flow cytometry (n=5). (E) Comparison of the expression of pluripotency markers in differently detached hES cells analysed by flow cytometry. After the detachment of hES cells, the cells were fixed, permeabilised and stained with antibodies detecting the pluripotency markers NANOG, OCT4, SOX2 and SSEA3. The data have been collected from four independent experiments and are presented as mean±s.d. (F) Changes in the availability of integrin β1 on the plasma membrane of hES cell after 72 h analysed by flow cytometry. The data have been collected from four independent experiments and are presented as mean±s.d.