Fig. 3.
Generation of the null allele Ror4. (A) The exon-intron structure of the Ror locus including the positions of the ATG and stop codons is shown. The P-element P{GSV3}GS8107 was first mobilized using P-transposase and re-integrated into the third exon of the Ror coding region (P{GSV3}GS8107-Hop). Then the region between the two P-elements was excised in a second transposase-mediated step. (B) Embryonic viability assay. Data were obtained by repeating each experiment at least three times. The error bars represent the standard deviation of the mean. *P<0.05; ***P<0.001 (independent samples Student's t-test).