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. 2018 Nov 22;9:2697. doi: 10.3389/fimmu.2018.02697

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Copyright © 2018 Jensen, Potts, Ready and Patterson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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General effects of PROTAC treatment on BV173 cells. (A) PROTAC treatment does not alter the amount of MHC-I on the cell surface: analyzed by flow cytometry and HLA-A2 staining (BB7.2), as well as, W6/32 (pan MHC-I, Figure S3). (B) MHC-I peptide length distribution from PROTAC-treated and DMSO-treated cells: PROTAC treatment does not change the length distribution of identified MHC-I peptides as evaluated for each PROTAC across all treatment time points and concentrations (aggregate values represented for each condition, plotted as fraction of total). (C) Gibbs clustering analysis revealed three predominant motifs present within isolated and identified HLA peptides. HLA alleles present in our tested cell line: HLA-A^*02:01, HLA-A^*30:01, HLA-B^*18:01, HLA-B^*15:10, HLA-C^*12:03, and HLA-C^*03:04.