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. 2018 Nov 28;15:31. doi: 10.1186/s12979-018-0136-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Gating strategy performed for NK cell subsets of the young (a), the old aged under 85 (b) and the oldest aged over 85 (c). Upper dot plots refer to lymphocyte gating - lymphocytes (G1) were gated within PBMC population based on FSC and SSC parameters. Lower dot plots refer to NK cell subsets gating: CD56dim (G2) and CD56bright (G3) cells were discriminated by low vs. high expression of CD56 within NK cell population defined as CD3 negative and CD56 positive cells (CD3-CD56+)