Figure 2.

n-NOS immunoreactivity in different brain regions of the rhesus monkey model of recurrent migraine (immunohistochemical staining).

Cells containing nNOS showed brownish-yellow granules under the microscope after staining. nNOS-positive cells were stained brown yellow in the nucleus. Repeated IS stimulation of the dura in rhesus monkeys increased the immunoreactivity of n-NOS in the parietal lobe (A1), thalamus (B1), hippocampus (C1) and pons (D1). (A2–D2) Images of the same brain regions from controls. Original magnification, 200×. (E) Plot of the counts of nNOS-positive cells in these four brain regions in the IS and control groups (*P < 0.05). Scale bars: 50 μm. nNOS: Neuronal nitric oxide synthase; IS: inflammatory soup.