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. Author manuscript; available in PMC: 2018 Nov 29.
Published in final edited form as: Virology. 2017 Jul 24;510:205–215. doi: 10.1016/j.virol.2017.07.023

Fig. 7. HCMV reactivation in T98G-LrV cells is associated with the PKA-CREB signaling pathway.

Fig. 7.

T98G-LrV cells were pretreated with H89 for 1 h, and then treated with cAMP/IBMX in the presence of H89 for 8 days. Medium was changed every 4 days. Supernatants and cells were collected for the subsequent assays. (A) Cell-free virus titer. Supernatants were subjected to the plaque forming assay to determine the cell-free virus. The results were presented as mean ± SD from 3 biological replicates. (B) Relative level of IE1. Total RNA was extracted from the collected cells, and relative transcription level of IE1 was examined by qPCR with GAPDH as the reference gene. *** p < 0.001. (C) Protein levels of IE1 and representative components of PKA-CREB signaling pathway. Cell lysates were prepared and equal amount of proteins were subjected to western blotting. The protein levels were quantitated by densitometry using Image J software (National Institutes of Health) and presented as the relative level to that of DMSO control after normalization to actin.