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. Author manuscript; available in PMC: 2018 Nov 29.
Published in final edited form as: Methods Enzymol. 2018 Jun 1;606:485–522. doi: 10.1016/bs.mie.2018.04.014

Fig. 4.

Fig. 4

Purification and reconstitution of S. aureus MoaA. (A) SDS-PAGE of MoaA at different purification steps. Lanes: 1, crude cell lysate; 2, Ni-NTA flow through; 3, Ni-NTA wash with 40mM imidazole; 4, Ni-NTA elution with 400mM imidazole; 5, after G-25; and 6, molecular weight marker. (B) UV–vis absorption spectra of MoaA (11.4μM) before (solid line) and after reconstitution (dashed line).