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. Author manuscript; available in PMC: 2018 Nov 29.
Published in final edited form as: Methods Enzymol. 2018 Jun 1;606:485–522. doi: 10.1016/bs.mie.2018.04.014

Fig. 5.

Fig. 5

Activity assays of MoaA. (A and B) Chemical derivatizations of 3′,8-cH2GTP to DMPT (A) and cPMP to compound Z (B). (C) Time course of the MoaA reaction in the absence of MoaC. The amount of 3′,8-cH2GTP formation was quantified by HPLC after its chemical derivatization to DMPT. (D) Time course of the formation of 5′-dA and cPMP during the catalysis in the coupled assay. cPMP was quantified by HPLC after its conversion to compound Z. Panels (C) and (D) are prepared using the previously published data (Hover et al., 2013).